Twitter said it is due to “the risk of further incitement of violence”.
“After close review of recent Tweets from the @realDonaldTrump account and the context around them we have permanently suspended the account due to the risk of further incitement of violence,” the social media giant said in a tweet.
A Wikipedia page listing Twitter’s most followed accounts, updated on January 8, 2021, does not include President Trump.
The account had been temporarily blocked following the riots at Capitol Hill by pro-Trump demonstrators on Wednesday.
He had initially been locked out of his account for 12 hours. The decision to permanently suspend the 45th US President comes 48 hours after the violent protests in Washington DC.
It comes as successor Joe Biden described him as “one of the most incompetent presidents in the history of the United States of America”.
Mr Biden believes it is a “good thing” Mr Trump will not be attending his inauguration on January 20.
Mr Trump has tweeted prolifically throughout his term, using the platform to quickly send messages out to the world.
Barack Obama, who preceded Mr Trump, remains the most popular account on Twitter, with 128 million followers.
On Trump’s decision to snub his Inauguration, Mr Biden said it is a “good thing”.
The President-elect added: “He is not fit to serve.
“He is one of the most incompetent presidents in the history of the United States of America.
“I was told on the way over here that he wasn’t going to show up at the inauguration – one of the few things he and I have ever agreed on.”
Thousands of Trump supporters witnessed his speech outside the White House on Wednesday in which he continued to refuse to concede defeat.
He told those in attendance: “We will never give up, we will never concede.”
At 1pm Eastern Time, pro-Trump protesters breached barriers around the Capitol Building, overwhelming officers in full riot gear.
By 3pm, officials had been evacuated and an armed standoff ensued.